What It Means If You Are Charged With DNA Evidence

  • how the DNA was deposited
  • when it was deposited
  • whether the contact relates to the alleged offence

A DNA database match occurs when a profile obtained from a crime scene is compared with profiles stored in the national DNA database (NCIDD).

A match does not mean the circumstances of the allegation are proven. It means that the genetic profile obtained from the sample is consistent with a profile already recorded in the system.

The NCIDD Database holds samples from:

  • Convicted persons
  • Suspects of current crimes
  • Forensic samples retained from earlier investigations
  • Volunteers

If a match is obtained is an investigative lead only. If prosecution proceeds in reliance upon the database match, an evidentiary reference sample must be obtained from the person of interest.

A reference DNA sample is taken from an individual. This might be used for comparison with DNA recovered from a crime scene or item.

This is usually obtained through:

  •  Buccal/mouth swab
  •  Blood sample

The purpose is not to determine guilt, but to allow scientists to assess whether a person can be included or excluded as a contributor to the DNA profile.

The comparison is statistical and does not explain how the DNA came to be present

Crime scene work is inherently difficult to control for contamination and DNA transfer.

Handling at scene can lead to the courts excluding DNA where contamination is a concern.

Scenes may be compromised from earlier policing or paramedic activity.

In some jurisdictions, uniformed general duty police may be handling items at scene.

In other jurisdictions, highly trained CSI officers will be conducting such work.

The way a sample is collected, stored, and handled can influence what is ultimately detected. DNA can transfer between people and objects and be present from earlier contact.

Certain matters require a detailed review of crime scene logs, witness statements and BWC camera footage to assess DNA anticontamination procedures applied in the matter

In sexual assault matters, items such as clothing and bedding are most commonly sampled by police within their facilities.

Following this, the samples are sent to the DNA lab for testing.

The movements of the evidentiary items prior to the sampling counter are also important.

Frequently complainants provide clothing sometime after the alleged incident, which can be problematic for DNA testing. Any DNA upon those items may be transferred from one location to another during handling and packaging.

An evidentiary review of the sampling will consider the sampling officers contemporaneous notes, statement, relevant complainant statement and Crown Case Statement

It is commonplace for the complainant to be medically examined in a sexual assault.

This process seeks to obtain background information and biological material associated with the allegation.

The process typically uses a specially designed kit for collection of samples by the medical officer, and these are known by various names across Australia, including SAIK, FMEK, FECK, or rape kit.

Broadly, the medical examiner might take swabs of skin or genitals, including internal areas.

These procedures must be taken with the utmost care to avoid DNA transfer from one location to another.

A review of the medical sampling will consider the medical officers contemporaneous notes and statement.

iLaboratory testing involves extracting DNA from the samples and generating a DNA profile.

The DNA profile (or profiles) are then evaluated further.

Biological fluid samples may also be subjected to various testing protocols.

The DNA interpretation process is complex and may involve:

  • Estimating the number of DNA contributors
  • Consideration of differential extraction lysis
  • Re-amplifying low level results
  • Creating composite DNA profiles
  • Comparing results to numerous ethnicity groups
  • Interpreting partial profiles
  • Running many statistical comparisons

In a typical matter, the DNA casefile generated is in the order of 200 pages (excluding the raw data).

nThe police investigative report is early evidence and might identify DNA and or body fluids.

The report information is used to target the investigation further and to support charges.

Body fluid results are often only presumptively tested and have not been confirmed by testing.

This report does not typically evidence the statistics (ie 100 billion times more likely).

Further DNA testing is often conducted after this report.

This report may describe:

  • Where samples were collected
  • Where DNA was identified
  • Who the DNA might originate from

This report is not a scientific interpretation and is unverified. This report will essentially be replaced by a lab DNA report for trial.

gThe laboratory report summarises the scientific results.

This report may be served very close to the trial date.

Further supplementary reports may be evidenced.

The report typically includes:

  • Whether the defendant can be included or excluded
  • There will frequently be unknown DNA contributors listed
  • The DNA likelihood ratio (ie 100 billion times more likely)
  • Biological fluids testing results

The full laboratory casefile will contain information about subjective decisions and assumptions applied.

Commonly, there is other testing in the DNA casefile which is not in the report.

The DNA casefile is not usually provided with the lab report.

The matter cannot be reviewed scientifically without the DNA casefile.

DNA evidence will essentially form part of trial, unless it’s admissibility is challenged.

The courts refuse DNA evidence entirely more often than many people might assume.

Refusal is nuanced, but might be for numerous reasons including complexity.

Exclusion may occur where:

  • DNA issues are too complex for a jury
  • The DNA cannot resolve the evidentiary question
  • The way the evidence is presented risks misunderstanding

The prospects of excluding DNA evidence in a particular matter are more closely aligned to the scientific elements.

Challenging the admissibility of the DNA evidence is both scientific and legal.

DNA is significant physical evidence, which might explain the allegation or another version

Studies acknowledge that jurors risk placing undue weight upon DNA evidence.

Undue weight can be increased where there is misunderstanding.

The court must be given DNA evidence that it understands.

The weight applied to DNA by the court depends on:

  • Whether there are issues with the results
  • How the interpretation is explained
  • How the DNA evidence is explained
  • How the DNA fits into background circumstances
  • Whether the DNA results support the allegation or not

Where DNA is central, it must be handled with the greatest precision.

DATABASE DNA MATCH

A DNA database match occurs when a profile obtained from a crime scene is compared with profiles stored in the national DNA database (NCIDD).

A match does not mean the circumstances of the allegation are proven. It means that the genetic profile obtained from the sample is consistent with a profile already recorded in the system.

The NCIDD Database holds samples from:

  • Convicted persons
  • Suspects of current crimes
  • Forensic samples retained from earlier investigations
  • Volunteers

If a match is obtained is an investigative lead only. If prosecution proceeds in reliance upon the database match, an evidentiary reference sample must be obtained from the person of interest.

REFERENCE DNA SAMPLE

A reference DNA sample is taken from an individual. This might be used for comparison with DNA recovered from a crime scene or item.

This is usually obtained through:

  •  Buccal/mouth swab
  •  Blood sample

The purpose is not to determine guilt, but to allow scientists to assess whether a person can be included or excluded as a contributor to the DNA profile.

The comparison is statistical and does not explain how the DNA came to be present

CRIME SCENE SAMPLES

Crime scene work is inherently difficult to control for contamination and DNA transfer.

Handling at scene can lead to the courts excluding DNA where contamination is a concern.

Scenes may be compromised from earlier policing or paramedic activity.

In some jurisdictions, uniformed general duty police may be handling items at scene.

In other jurisdictions, highly trained CSI officers will be conducting such work.

The way a sample is collected, stored, and handled can influence what is ultimately detected. DNA can transfer between people and objects and be present from earlier contact.

Certain matters require a detailed review of crime scene logs, witness statements and BWC camera footage to assess DNA anticontamination procedures applied in the matter

CRIME ITEM SAMPLES

In sexual assault matters, items such as clothing and bedding are most commonly sampled by police within their facilities.

Following this, the samples are sent to the DNA lab for testing.

The movements of the evidentiary items prior to the sampling counter are also important.

Frequently complainants provide clothing sometime after the alleged incident, which can be problematic for DNA testing. Any DNA upon those items may be transferred from one location to another during handling and packaging.

An evidentiary review of the sampling will consider the sampling officers contemporaneous notes, statement, relevant complainant statement and Crown Case Statement

MEDICAL EXAMINATION

It is commonplace for the complainant to be medically examined in a sexual assault.

This process seeks to obtain background information and biological material associated with the allegation.

The process typically uses a specially designed kit for collection of samples by the medical officer, and these are known by various names across Australia, including SAIK, FMEK, FECK, or rape kit.

Broadly, the medical examiner might take swabs of skin or genitals, including internal areas.

These procedures must be taken with the utmost care to avoid DNA transfer from one location to another.

A review of the medical sampling will consider the medical officers contemporaneous notes and statement.

LAB TESTING

iLaboratory testing involves extracting DNA from the samples and generating a DNA profile.

The DNA profile (or profiles) are then evaluated further.

Biological fluid samples may also be subjected to various testing protocols.

The DNA interpretation process is complex and may involve:

  • Estimating the number of DNA contributors
  • Consideration of differential extraction lysis
  • Re-amplifying low level results
  • Creating composite DNA profiles
  • Comparing results to numerous ethnicity groups
  • Interpreting partial profiles
  • Running many statistical comparisons

In a typical matter, the DNA casefile generated is in the order of 200 pages (excluding the raw data).

POLICE DNA REPORT

nThe police investigative report is early evidence and might identify DNA and or body fluids.

The report information is used to target the investigation further and to support charges.

Body fluid results are often only presumptively tested and have not been confirmed by testing.

This report does not typically evidence the statistics (ie 100 billion times more likely).

Further DNA testing is often conducted after this report.

This report may describe:

  • Where samples were collected
  • Where DNA was identified
  • Who the DNA might originate from

This report is not a scientific interpretation and is unverified. This report will essentially be replaced by a lab DNA report for trial.

LAB DNA REPORT

gThe laboratory report summarises the scientific results.

This report may be served very close to the trial date.

Further supplementary reports may be evidenced.

The report typically includes:

  • Whether the defendant can be included or excluded
  • There will frequently be unknown DNA contributors listed
  • The DNA likelihood ratio (ie 100 billion times more likely)
  • Biological fluids testing results

The full laboratory casefile will contain information about subjective decisions and assumptions applied.

Commonly, there is other testing in the DNA casefile which is not in the report.

The DNA casefile is not usually provided with the lab report.

The matter cannot be reviewed scientifically without the DNA casefile.

EXCLUSION OF DNA EVIDENCE

DNA evidence will essentially form part of trial, unless it’s admissibility is challenged.

The courts refuse DNA evidence entirely more often than many people might assume.

Refusal is nuanced, but might be for numerous reasons including complexity.

Exclusion may occur where:

  • DNA issues are too complex for a jury
  • The DNA cannot resolve the evidentiary question
  • The way the evidence is presented risks misunderstanding

The prospects of excluding DNA evidence in a particular matter are more closely aligned to the scientific elements.

Challenging the admissibility of the DNA evidence is both scientific and legal.

TRIAL

DNA is significant physical evidence, which might explain the allegation or another version

Studies acknowledge that jurors risk placing undue weight upon DNA evidence.

Undue weight can be increased where there is misunderstanding.

The court must be given DNA evidence that it understands.

The weight applied to DNA by the court depends on:

  • Whether there are issues with the results
  • How the interpretation is explained
  • How the DNA evidence is explained
  • How the DNA fits into background circumstances
  • Whether the DNA results support the allegation or not

Where DNA is central, it must be handled with the greatest precision.

  • may be a contributor, or
  • is excluded as a contributor.
  • How items were handled before sampling
  • Whether samples were contaminated
  • What items were sampled and where
  • How many DNA contributors in a DNA profile
  • What methods are used to test body fluids
  • Whether body fluids are confirmed
  • Whether body fluids can be linked to DNA or not
  • Social contact between parties
  • How results are reported
  • Whether contact occurred at all
  • Whether the DNA got there recently
  • Whether DNA got there by touching
  • Whether the DNA got there without any contact
  • How likely the allegations are given the DNA results
  • The body
  • Clothing
  • Bedding
  • Shared environments

Common DNA Evidence Issues in Criminal Cases

DNA can be deposited when we touch, sneeze, bleed, sweat, cough or even talk.

The term “touch DNA” can be erroneously applied, because we simply can’t say whether touch occurred.

The term has been replaced with “trace DNA”, meaning DNA which cannot be linked to a particular body fluid.

Trace DNA profiles are common in evidence, because skin cell DNA is common, and it can be difficult to determine whether DNA is from a body fluid.

Trace DNA can be present through:

  • Direct contact
  • Indirect contact
  • Shared environments

The presence of trace DNA does not indicate whether contact occurred or when the DNA got there.

Saliva is frequently relied upon in criminal matters in support of various allegations.

Saliva testing is known to cross react with various substance including vaginal material, urine and sweat.

A positive saliva test result may be informative in some contexts, but it does not automatically explain:

  • Whether saliva is even present
  • How the material was deposited
  • When the material was deposited
  • Who the material originates from
  • Whether it relates to the alleged event

Whilst significant issues exist with saliva testing, it should not be presumed that it has no evidentiary value.

Any saliva test positive result should be evaluated carefully in context of the matter.

A positive semen result generally relates to a presumptive test.

It is critical to understand whether semen is truly confirmed.

Confirmation can be conducted through a microscope observation of sperm cells.

A high number of sperm cells might be consistent with a full healthy ejaculate in one matter.

A low number of sperm cells might be consistent with semen transfer in another matter.

Evaluation of semen would consider:

  • The number of sperm cells
  • whether semen is consistent with ejaculation
  • What can be understood about timing
  • Whether semen is confirmed
  • Whether the semen can be attributed to an individual

Any semen result is highly scientific and must be reviewed precisely.

Careful scientific review should be applied to any matter involving semen results.

Consideration of blood can rely on visual appearance, which can be problematic.

Reported positive blood often relates to a presumptive test.

This might be because insufficient quantities exist for confirmatory testing.

The significant evidentiary question can be whether the DNA originates from the blood (ie: whose blood is this)

Matching blood to a particular donor can be difficult, particularly as mixed DNA profiles are common.

 

Review of blood evidence would consider:

  • Is the evidenced stain appearance consistent with blood
  • Is testing presumptive and how reliable is that result
  • Can the stain be linked to a particular donor
  • Might the stain be consistent with direct or indirect deposit

Certain matters featuring blood may also lend themselves to bloodstain pattern analysis, which might assist with clarifying certain activity related to the staining.

It can be critical to prosecution and defence “whose semen” it is.

To consider “whose semen” the lab will conduct what is called a differential extraction.

The process is not always successful, and it is inherently scientific to interpret.

When reading an expert report with a differential extraction, you will see a “sperm fraction” and a “non-sperm” fraction.

Surprisingly, the “sperm fraction” does not necessarily contain sperm.

Even more surprisingly, the “sperm fraction” and “non-sperm” fraction can be reported when there is no sperm present.

Any differential extraction should be reviewed by a DNA expert.

DNA recovered from clothing and bedding may arise from:

  • Direct contact
  • No contact
  • Secondary transfer
  • Shared environments
  • Earlier handling
  • Later handling
  • Laundering

The presence of DNA on clothing and bedding does not necessarily indicate when it was deposited or what activity led to its presence.

Interpretation is more than what is merely possible. To consider the DNA profile scientifically, one must evaluate the case background, handling, seizure and sampling applied. BWC footage is becoming an increasingly common inclusion with review of these factors.

TOUCH DNA

DNA can be deposited when we touch, sneeze, bleed, sweat, cough or even talk.

The term “touch DNA” can be erroneously applied, because we simply can’t say whether touch occurred.

The term has been replaced with “trace DNA”, meaning DNA which cannot be linked to a particular body fluid.

Trace DNA profiles are common in evidence, because skin cell DNA is common, and it can be difficult to determine whether DNA is from a body fluid.

Trace DNA can be present through:

  • Direct contact
  • Indirect contact
  • Shared environments

The presence of trace DNA does not indicate whether contact occurred or when the DNA got there.

SALIVA

Saliva is frequently relied upon in criminal matters in support of various allegations.

Saliva testing is known to cross react with various substance including vaginal material, urine and sweat.

A positive saliva test result may be informative in some contexts, but it does not automatically explain:

  • Whether saliva is even present
  • How the material was deposited
  • When the material was deposited
  • Who the material originates from
  • Whether it relates to the alleged event

Whilst significant issues exist with saliva testing, it should not be presumed that it has no evidentiary value.

Any saliva test positive result should be evaluated carefully in context of the matter.

SEMEN

A positive semen result generally relates to a presumptive test.

It is critical to understand whether semen is truly confirmed.

Confirmation can be conducted through a microscope observation of sperm cells.

A high number of sperm cells might be consistent with a full healthy ejaculate in one matter.

A low number of sperm cells might be consistent with semen transfer in another matter.

Evaluation of semen would consider:

  • The number of sperm cells
  • whether semen is consistent with ejaculation
  • What can be understood about timing
  • Whether semen is confirmed
  • Whether the semen can be attributed to an individual

Any semen result is highly scientific and must be reviewed precisely.

Careful scientific review should be applied to any matter involving semen results.

BLOOD

Consideration of blood can rely on visual appearance, which can be problematic.

Reported positive blood often relates to a presumptive test.

This might be because insufficient quantities exist for confirmatory testing.

The significant evidentiary question can be whether the DNA originates from the blood (ie: whose blood is this)

Matching blood to a particular donor can be difficult, particularly as mixed DNA profiles are common.

 

Review of blood evidence would consider:

  • Is the evidenced stain appearance consistent with blood
  • Is testing presumptive and how reliable is that result
  • Can the stain be linked to a particular donor
  • Might the stain be consistent with direct or indirect deposit

Certain matters featuring blood may also lend themselves to bloodstain pattern analysis, which might assist with clarifying certain activity related to the staining.

WHOSE SEMEN?

It can be critical to prosecution and defence “whose semen” it is.

To consider “whose semen” the lab will conduct what is called a differential extraction.

The process is not always successful, and it is inherently scientific to interpret.

When reading an expert report with a differential extraction, you will see a “sperm fraction” and a “non-sperm” fraction.

Surprisingly, the “sperm fraction” does not necessarily contain sperm.

Even more surprisingly, the “sperm fraction” and “non-sperm” fraction can be reported when there is no sperm present.

Any differential extraction should be reviewed by a DNA expert.

DNA ON CLOTHING

DNA recovered from clothing and bedding may arise from:

  • Direct contact
  • No contact
  • Secondary transfer
  • Shared environments
  • Earlier handling
  • Later handling
  • Laundering

The presence of DNA on clothing and bedding does not necessarily indicate when it was deposited or what activity led to its presence.

Interpretation is more than what is merely possible. To consider the DNA profile scientifically, one must evaluate the case background, handling, seizure and sampling applied. BWC footage is becoming an increasingly common inclusion with review of these factors.

  • Evaluating how samples were selected and collected (review police sampling)
  • Reviewing the biological fluid testing
  • Reviewing medical records relating to collection of intimate samples
  • Assessing the nature of any DNA mixed profiles (Review electropherograms)
  • Considering transfer and persistence (may include BWC footage review)
  • Evaluating the statistical reporting (review STRmix extended outputs)
  • Placing the results within the factual context of the case (review witness statements and background)
  • Presentation of scientific data relevant to the matter
  • Make informed decisions in discussion with your lawyer
  • Appreciate the strengths and limits of the scientific material
  • Ask the right questions about the evidence presented
  • Recognise when further independent review could be valuable
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Helen Roebuck DNA expert giving evidence

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